ETHIDIUM HOMODIMER I SOLUTION 2MM IN DMSO

Application

Ethidium homodimer I solution has been used to check membrane integrity in sperms.

Stains ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA structures.

Used to detect DNA in solution.

Biochem/physiol Actions

Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity.

One molecule of EthD-1 will non-selectively bind four base pairs in dsDNA.

Molecular Weight: 856.75. Empirical Formula: C₄₆H₅₀Cl₄N₈. Quality Level: 100. Assay: ≥. 95%. form: liquid. concentration: 2 . mM in DMSO. color: red. ε (extinction coefficient): ≥. 9000 at 490-496 . nm in water. application(s): diagnostic assay manufacturing
hematology
histology. storage temp.: −. 20°C. SMILES string: Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28. InChI: 1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32. . . . /h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2. 4*1H. InChI key: ZKAJUKABUUJRLB-UHFFFAOYSA-N. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): 185.0 °F - closed cup. Flash Point(C): 85 °C - closed cup. Personal Protective Equipment: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US).