ECL WESTERN BLOTTING ANALYSIS SYSTEM

General description

ECL^™ Western Blotting System is the world′s most widely used and referenced chemiluminescent immunodetection system.

ECL^™ Western Blotting System uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL^™ Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.

Each system contains the following reagents, sufficient for detection of 1000 cm² membrane:
Anti-mouse HRP conjugate, 100 μl
Anti-rabbit HRP conjugate, 100 μl
ECL^™ Detection Reagents 1 and 2, 62.5 mL each
Blocking agent, 5 g.

Application

ECL^™ Blotting Analysis System has been used in western blotting.

Western Blotting

Features and Benefits

• Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL^™ reagents.
• Optimized system provides rapid, sensitive results using simple protocols.
• Designed for use with nitrocellulose and PVDF membranes.
• High sensitivity: Up to 10 times more sensitive than colorimetric methods.
• Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.
• Easy reprobing: Save time and materials by reprobing on membranes up to 10 times.
• Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,
• Non-radioactive-no specialized facilities needed
• Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

ECL is a trademark of Cytiva

shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.. feature: wetted part: no. packaging: pkg of 1 . kit. manufacturer/tradename: Cytiva RPN2108. color: Detection reagent 1: Colorless Detection reagent 2: Colorless Peroxidase labelled anti-mouse antibody: Colorless Peroxidase labelled anti-rabbit antibody: Colorless Blocking agent: White. storage temp.: 2-8°C. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H317. Precautionary Statements: P101 - P102 - P103 - P261 - P272 - P280 - P302 + P352 - P333 + P313 - P501. Storage Class Code: 12 - Non Combustible Liquids.