General description
Dulbecco′s Modified Eagle′s medium (DMEM), a regularly used cell culture medium, is a source of energy-rich media that includes glucose, vitamins, and amino acids. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used in cell culture research. To compensate for the loss of buffering capacity caused by removing serum, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer may be added in the formulations at a final concentration of 15 mM.
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham:
- has been used as a component in the culture medium to culture primary culture-activated human hepatic stellate cells
- has been used to culture the cartilage biopsies
- may be used for the propagation of Cowdria ruminantium
Quality Level: 400. sterility: sterile-filtered. form: liquid. technique(s): cell culture | mammalian: suitable. impurities: endotoxin, tested. web metadata keyword.default: classic media, classical media, serum supplement, FBS, high glucose. components: glucose: 3.15 . g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 . g/L. shipped in: ambient. storage temp.: 2-8°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.
- UPC:
- 10171505
- Condition:
- New
- HazmatClass:
- No
- MPN:
- D8437-500ML
akash.verma@cenmed.com
(732) 447-1115





