DMEM HIGH MEDIUM W/ L-GLUT. 1L (C005B-113042)

General description

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Quality Level: 400. sterility: sterile-filtered. form: liquid. technique(s): cell culture | mammalian: suitable. impurities: endotoxin, tested. web metadata keyword.default: DMEM, DMEM-high glucose, DMEM/High, Dulbeccos-MEM-high glucose, Dulbeccos-MEM/High, DME high glucose, DME/High, Dulbeccos Modified Eagles Medium - high glucose, DME, DMEM/High, adipocytes, chondrocytes, epithelial, cell culture, Dulbecco′. s. components: NaHCO₃: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no. shipped in: ambient. storage temp.: 2-8°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.