DNA TEMPLATE EXTRACTION, 100

OmniTemplateTM is specifically designed for the rapid isolation of a DNA template for polymerase chain reaction (PCR) analysis from mammalian tissue samples, blood and cell cultures. This single tube method produces a high concentration, ready-to-use supply of DNA template for microanalysis, genotyping and a multitude of other applications. The method involves isolation of nuclei and their subsequent digestion with LongLife TM Proteinase K in the same tube the samples were collected in. The isolated nuclei are digested to release template DNA in a buffer compatible with PCR and other downstream applications. The OmniTemplateTM samples can be directly used in PCR and other applications without further manipulations. One prep is approximately 1-10mg tissue. Figure: OmniTemplateTM produces high quality genomic DNA template. 5μl (lanes 1, 3, 5) or 15μl (lanes 2, 4, 6) PCR reactions were loaded onto a 2% agarose gel. Template was purified from 10 4 (lanes 1-2), 10 6 (lanes 3-4) or 2.8x10 6 (lanes 5-6) NIH3T3 cells. Features Single tube genomic DNA isolation. Compatible with PCR and other downstream applications. Suitable for blood, cells, and animal tissue samples. No toxic chemicals, no phenol, no hazardous waste. Applications Designed for the rapid preparation of DNA template for large throughput microanalysis. High recovery of ready-to-use DNA templates in a short period of time.