DNA MB-GRADE

General description

DNA, MB-grade is used for the prevention of nonspecific hybridization reactions.

DNA, MB-grade, is provided as a sonicated, denatured single-stranded DNA fragment mixture.

Application

The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.

Sequence

Chain Length 120 to 3,000 nucleotides

Physical form

Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0

Preparation Note

Working concentration: 100μg/ml
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Quality Level: 100. form: solution (ready-to-use). mol wt: 40-1,000 . kDa. packaging: pkg of 500 . mg. manufacturer/tradename: Roche. storage temp.: −. 20°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: nwg. Flash Point(F): No data available. Flash Point(C): No data available.