DNA GYRASE E. COLI

Application

DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation.

Biochem/physiol Actions

DNA gyrase is supplied as an A₂B₂ holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA. DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments.

Can be used to supercoil plasmids.

Unit Definition

One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37 °C.

Other Notes

Solution in 50% Glycerol containing Tris buffer, DTT and EDTA.

form: aqueous glycerol solution. Quality Level: 200. mol wt: ~374 . kDa. concentration: ≥. 2 . unit/μ. L. UniProt accession no.: P0AES4, P0AES6. shipped in: dry ice. storage temp.: −. 70°C. Gene Information: Escherichia coli K12 ... gyrA(946614), gyrB(948211). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.