DMT-2''O-TBDMS-RA(BZ) AMIDITE SEPTUM (C005B-005492)

General description

RNA phosphoramidites, along with RNA CPG, and analogous to DNA synthesis support the synthesis of sequence-specific RNA oligonucleotides for RNA interference (RNAi) and can be used in target validation and other drug development techniques. Modern phosphoramidite-mediated synthesis has enabled routine high-yielding preparations of RNA oligonucleotides. High-quality RNA phosphoramidites are key to low failure rates, the high biological activity of synthesis products, and cost-effectiveness.

Key Features

• Industry-standard 2′O-TBDMS protective group
• Consistent lot-to-lot purity and performance
• Compatible with deprotection methods based on methylamine or AMA
• Standard RNA phosphoramidites provide excellent coupling results when used with ETT or BTT as an activator; best results are obtained with activator 42
• Capping with standard acetic anhydride capping reagent rather than with fast Deprotection Cap A
• Manufactured under a certified ISO 9001 quality system

DMT-2′O-TBDMS-rA(bz) Phosphoramidite is configured for ÄKTA and OligoPilot Synthesizers.

Legal Information

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Molecular Weight: 988.19. Empirical Formula: C₅₃H₆₆N₇O₈PSi. biological source: non-animal source (no BSE/TSE risk). Quality Level: 200. product line: Proligo Reagents. Assay: ≥. 99% (³¹P-NMR), ≥. 99.0% (reversed phase HPLC). form: powder. technique(s): oligo synthesis: suitable. impurities: ≤. 0.3% water content (Karl Fischer), ≤. 0.5% P(III) Impurities 100-169ppm (³¹P-NMR), ≤. 0.5% single Impurity (reversed phase HPLC), ≤. 3% residual Solvent content. color: white to off-white. λ: conforms (UV/VIS Identity). suitability: conforms to structure for H-NMR, conforms to structure for LC-MS. compatibility: configured for (ÄKTA^®. and OligoPilot^®. ). nucleoside profile: base: adenosine
base protecting group: benzoyl
2' protecting group: TBDMS
5' protecting group: DMT
deprotection: standard. storage temp.: −. 20°C. SMILES string: COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](O[Si](C)(C)C(C)(C)C)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4c(NC(: O)c5ccccc5)ncnc34)(c6ccccc6)c7ccc(OC)cc7. InChI: 1S/C53H66N7O8PSi/c1-36(2)60(37(3)4)69(65-32-18-31-54)67-46-44(33-64-53(39-21-16-13-17-22-39,40-23-27-42(62-8)28-24-40)41-25-29-43(63-9)30-26-41)66-51(47(46)68-70(10,11)52(5,6)7)59-35-57-45-48(55-34-56-49(45)59)58-50(61)38-19-14-12-15-20-38/h12-17,19-30,34-37,44,46-47,51H,18,32-33H2,1-11H3,(H,55,56,58,61)/t44-,46-,47-,51-,69?/m1/s1. InChI key: FFXHNCNNHASXCT-RFMFGJHUSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.