DIRECTLOAD(TM) 50 BP DNA STEP LADDER READY-TO-USE MARKER FOR DNA ELECTROPHORESIS

General description

Sigma′s DirectLoad^™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

DirectLoad^™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

• Ready-to-load
• Easy-to-use
• Popular band sizes

Components

Sigma′s DirectLoad^™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Quality Level: 200. form: (liquid). usage: sufficient for 100 . loads. suitability: suitable for electrophoresis (DNA). web metadata keyword.default: NA electrophoresis, ladder, marker. storage temp.: −. 20°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.