DENHARDT'S SOLUTION POWDER FOR BLOCKING HYBRIDIZATION BLOTS (C005B-113227)

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Application

Denhardt′s Solution has been used:

• as a component in hybridization buffer for the hybridization of brain sections
• in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISH_PAP-WM) process
• in the preparation of hybridization mixture

Suitable as a blocking Buffer for Southern and Northern blot membranes.

Biochem/physiol Actions

Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.

Components

Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).

Preparation Note

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.

grade: for molecular biology. Quality Level: 100. form: lyophilized powder. technique(s): Southern blotting: suitable. foreign activity: DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected. storage temp.: 2-8°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).