DEMECOLCINE (C005B-041923)

Application

Demecolcine has been used as a microtubule depolymerizing agent. It has also been used to inhibit mitotic division.

Biochem/physiol Actions

Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G₁ and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Molecular Weight: 371.43. Empirical Formula: C₂₁H₂₅NO₅. Quality Level: 200. Assay: ≥. 98% (HPLC). form: powder. mp: 184 . °C. solubility: chloroform: 10 . mg/mL, ethanol: soluble. application(s): diagnostic assay manufacturing
hematology
histology. storage temp.: 2-8°C. SMILES string: CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3: CC: C(OC)C(: O)C: C13. InChI: 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1. InChI key: NNJPGOLRFBJNIW-HNNXBMFYSA-N. Pictograms: GHS06. Signal Word: Danger. Hazard Statements: H300. Precautionary Statements: P264 - P270 - P301 + P310 - P405 - P501. Hazard Classifications: Acute Tox. 2 Oral. Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges.