DCTP PCR GRADE DI-NA SLT

General description

dCTP, PCR Grade, is a 100 mM clear colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Application

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

Quality

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Physical form

solution of dCTP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Other Notes

For general laboratory use.

Molecular Weight: 511.12. Empirical Formula: C₉H₁₄N₃Na₂O₁₃P₃. description: PCR Grade, sodium salt. Quality Level: 100. Assay: 99% (dNTP). form: solution. usage: sufficient for 125,000 . standard reactions (03732690001), sufficient for 31,250 . standard reactions (11969021001), sufficient for 6,250 . standard reactions (11934520001). packaging: pkg of 1,250 . μ. L (11969021001 [100 mM]), pkg of 4 × 1,250 . μ. L (03732690001 [4x100 mM]), pkg of 250 . μ. L (11934520001 [100 mM]). manufacturer/tradename: Roche. concentration: <. 0.9% (dNDP), 100 . mM. storage temp.: −. 20°C. SMILES string: [Na+].[Na+].NC1: NC(: O)N(C: C1)[C@H]2C[C@H](O)[C@@H](COP(O)(: O)OP([O-])(: O)OP(O)([O-]): O)O2. InChI: 1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17. . /h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17). . /q. 2*+1/p-2/t5-,6+,8+. . /m0../s1. InChI key: ABWVCNMFYVEBIB-CDNBRZBRSA-L. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): does not flash. Flash Point(C): does not flash.