DANSYL LABELED POLYMYXIN B READY MADE SOLUTION

General description

Dansyl Polymyxin B (DSL-PMB) is a fluorescent derivative of Polymyxin B. Polymyxin B is a cationic lipopeptide antibiotic that binds specifically to Gram-negative bacteria. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore.

Dansyl labeled Polymyxin B can be utilized for fluorescent microbial imaging in the fields of Cell Biology and Biochemical research.

Application

DSL-PMB may have been used:

• as a fluorescent probe to study polymyxin mode of action and its pharmacokinetics
• in research and to develop new active derivatives of Polymyxin against multi-drug resistance Gram-negative infections
• to measure LPS binding affinity by fluorometric displacement assay

• Antimicrobial resistance research.
• Bacterial visualization and imaging.
• Parent antibiotic mode of action research and new antibiotic discovery.
• Toxicity studies.
• Research of bacterial infections and tracking its uptake in vivo.

Biochem/physiol Actions

DSL-PMB′s mode of action is in accordance with Polymyxin B activity. However, MIC assays comparing DSL-PMB, and parent Polymyxin B showed a two to three-fold decrease in bacterial activity. Polymyxin B interacts with anionic lipopolysaccharide (LPS) molecules which are located in Gram-negative bacterial outer membrane. This interaction interferes with the outer membrane′s normal function, causing leakage and enhanced uptake of the antibiotic. The resemblance in properties between Polymyxin B and DSL-PMB allows to fluorescently label Gram-negative bacteria and examine intracellular localization and penetration of Polymyxins in Gram-negative bacteria.

Features and Benefits

• High-quality antibiotic suitable for multiple research applications
• Ideal for Cell Biology, Metabolomics, and Biochemical research.

Analysis Note

• Dansyl Labeled Polymyxin B Ready Made Solution is light sensitive.
• It is recommended to avoid freeze-thaw cycles of PMB-DSL Ready Made Solution.
• Dansyl Labeled Polymyxin B Ready Made Solution (1.5 mg/mL) can be diluted 1:50 in PBSX1 (Sigma#D8537) to achieve 30 μg/mL final concentration for staining. The above concentration of DSL-PMB was used for E. coli staining see image.
• Fluorescence Microscopy application: Dansyl Labeled Polymyxin B Ready Made Solution excitation (Ex) wavelength is 330-340nm resulting in emission (Em) range of 540-600nm (λ_max=570nm).