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daf-fm

DAF-FM

Catalog No.
C005B-027916
Manufacturer No.
D1821-1MG
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
MG

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Application DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.

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Application

DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.

Biochem/physiol Actions

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

Caution

hygroscopic

Preparation Note

Color variation within the range from white to brown does not change performance.

Molecular Weight: 412.34. Empirical Formula: C21H14F2N2O5. Quality Level: 100. Assay: ≥. 98% (HPLC). form: powder. storage condition: desiccated. color: white to brown. solubility: DMSO: >. 1 . mg/mL. shipped in: wet ice. storage temp.: −. 20°C. SMILES string: CNc1ccc2c(c1N)C(: O)OC23c4cc(F)c(O)cc4Oc5cc(O)c(F)cc35. InChI: 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3. InChI key: DIJCILWNOLHJCG-UHFFFAOYSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.
UPC:
12141802
Condition:
New
HazmatClass:
No
MPN:
D1821-1MG
CAS:
254109-20-1
Temperature Control Device:
Yes

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