D(+)-TREHALOSE DIHYDRATE FOR MICROBIOLOGY (C005B-052778)

General description

D-(+)-Trehalose is a disaccharide composed of two α-glucose units. It is used as a cryoprotectant in a variety of cell freezing media. Used as a protectant in transportation media. It predominantly exists among the species that support anhydrobiosis (life without water). It serves as a carbohydrate reserve in microorganisms and protects them from adverse conditions, such as heat, cold, dehydration, desiccation, and oxidation. Trehalose acts as a chemical chaperone affecting protein folding. It is known to prevent the generation of amyloids and accumulation of β-amyloid in vitro. A similar effect of trehalose has been observed with Huntingtin protein aggregation. Thus, it might serve as a protective agent in Alzheimer′s disease and Huntington′s disease. Trehalose stimulates the better initial growth of Salmonella cells.

Application

D-(+)-Trehalose can be used as a carbohydrate reserve in certain cell cultures to tide over environmental stresses. It can also be used in biopharmaceutical monoclonal antibody formulations and as a protein stabilizer.

Molecular Weight: 378.33. Empirical Formula: C₁₂H₂₂O₁₁ · 2H₂O. Quality Level: 200. Assay: ≥. 99.0% (HPLC), ≥. 99.0%. form: powder. optical activity: [α. ]20/D +176 to +186°, c :  . 2% in H₂O. mol wt: 378.33  . g/mol. impurities: ≤. 0.1% glucose. ign. residue: ≤. 0.1% (as SO₄). solubility: H₂O: 0.1 . g/mL, clear, colorless. anion traces: chloride (Cl^-): ≤. 50 . mg/kg, sulfate (SO₄^2-): ≤. 50 . mg/kg. cation traces: As: ≤. 0.1 . mg/kg, Ca: ≤. 200 . mg/kg, Cd: ≤. 5 . mg/kg, Co: ≤. 5 . mg/kg, Cr: ≤. 5 . mg/kg, Cu: ≤. 5 . mg/kg, Fe: ≤. 5 . mg/kg, K: ≤. 50 . mg/kg, Mg: ≤. 10 . mg/kg, Mn: ≤. 5 . mg/kg, Na: ≤. 50 . mg/kg, Ni: ≤. 5 . mg/kg, Pb: ≤. 5 . mg/kg, Zn: ≤. 10 . mg/kg. application(s): microbiology. SMILES string: [H]O[H].[H]O[H].OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O. InChI: 1S/C12H22O11.2H2O/c13-1-3-5(15)7(17)9(19)11(21-3)23-12-10(20)8(18)6(16)4(2-14)22-12. . /h3-20H,1-2H2. 2*1H2/t3-,4-,5-,6-,7+,8+,9-,10-,11-,12-. . /m1../s1. InChI key: DPVHGFAJLZWDOC-PVXXTIHASA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).