CYCLOPENTYLACETYLENE 90% (C005B-089184)

General description

A reversible inhibitor of trypsin that binds to trypsin in a stoichiometric, pH-dependent manner (K_d = 500 nM at pH 7.8).

Reversible inhibitor of trypsin that binds to trypsin in a stoichiometric, reversible and pH-dependent manner (K_d = 0.5 nM at pH 7.8). Also inhibits, to a lesser degree, chymotrypsin, kallikrein, plasmin, and other serine proteases.

Biochem/physiol Actions

Kd = 500 nM at pH 7.8 against trypsin

Primary Target
trypsin

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of protein that will inhibit 1 unit of trypsin activity using BAEE as a substrate at 25°C, pH 7.6.

Reconstitution

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 week at 4°C.

Other Notes

Lilly, C.M., et al. 1993. J. Clin. Invest.91, 235.
Uchino, T., et al. 1993. J. Biol. Chem.268, 527.
Birk, Y. 1976. Methods Enyzmol.45, 700.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

form: lyophilized solid. Quality Level: 100. specific activity: ≥. 4000 . units/mg protein. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, desiccated. color: off-white. solubility: 67 mM potassium phosphate buffer, pH 7.6: 5 . mg/mL. storage temp.: −. 20°C. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H317 - H334. Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313. Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.