CRYSTAL VIOLET CERTIFIED BY THE BIOLOGICAL STAIN COMMISSION (C005B-048181)

Application

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Suitability

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.

Molecular Weight: 407.98. Empirical Formula: C₂₅H₃₀ClN₃. linearFormula: C₂₅H₃₀N₃Cl. grade: certified by the Biological Stain Commission. Quality Level: 200. form: powder. composition: Dye content, ≥. 90% . mp: 205 . °C (dec.) (lit.). solubility: H₂O: 1 . mg/mL. density: 1.19 . g/cm³ . at 20 . °C. antibiotic activity spectrum: fungi. application(s): diagnostic assay manufacturing
hematology
histology. Mode of action: cell membrane | interferes, enzyme | inhibits. storage temp.: room temp. SMILES string: [Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C): C3/C: C\C(C: C3): [N+](/C)C. InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6. /h7-18H,1-6H3. 1H/q+1. /p-1. InChI key: ZXJXZNDDNMQXFV-UHFFFAOYSA-M. Pictograms: GHS05,GHS07,GHS08,GHS09. Signal Word: Danger. Hazard Statements: H302 - H318 - H351 - H410. Precautionary Statements: P202 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313. Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.