COMPLEMENT C3 HUMAN

General description

Native, human C3 complement component. Glycoprotein composed of two non-identical disulfide-bonded subunits with M.W. of 115 kDa (α) and 75 kDa (β). Present in normal human serum at 1.25 mg/ml. Classical and alternative activation pathways of complement converge at C3 step. Activation via either pathway can result in assembly of C3-cleaving enzymes (C3 convertases) on target surfaces. Both C3 convertases cleave the C3 α-chain at peptide bond 77 resulting in production of C3a (M.W. 9083) and C3b fragments (M.W. 180,000). Released C3a peptide is one of the three complement anaphylatoxins. The nascent C3b fragment can form a covalent ester bond with target surface. This covalent attachment of C3b to target acceptors is required for continuation of complement activation.

Native, human C3 complement component. Glycoprotein composed of two non-identical disulfide-bonded subunits with M.W. of 115,000 (α) and 75,000 (β). Present in normal human serum at 1.25 mg/ml. Classical and alternative activation pathways of complement converge at the C3 step. Activation via either pathway can result in assembly of C3-cleaving enzymes (C3 convertases) on target surfaces. Both C3 convertases cleave the C3 α-chain at peptide bond 77, resulting in production of C3a (M.W. 9083) and C3b fragments (M.W. 180,000). Released C3a peptide is one of the three complement anaphylatoxins. The nascent C3b fragment can form a covalent ester bond with target surface. The covalent attachment of C3b to target acceptors is required for continuation of complement activation.

Biochem/physiol Actions

>3,000 C₃H₅0 units/mg; ≥60% of the C3 activity in normal human serum on a mg/mg basis.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS.

Preparation Note

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Lambris, J.D. and Muller-Eberhard, H.J. 1986. Mol. Immunol.23, 1237.
Tack, B.F., et al. 1980. Methods Enzymol. 80, 64.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level: 100. Assay: ≥. 85% (SDS-PAGE). form: liquid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. impurities: IgG, IgA, IgM, C4, functionally active C5, albumin or ceruloplasmin, trace. shipped in: wet ice. storage temp.: −. 70°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.