COLLAGEN FROM HUMAN PLACENTA BORNSTEIN AND TRAUB TYPE IV (C005B-088477)

Biochem/physiol Actions

During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.

Preparation Note

This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It is prepared by a modification of the pepsin extraction method of Glanville, R.W., et al.

Analysis Note

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

biological source: human placenta. Quality Level: 200. description: Sigma Type VI. form: powder. technique(s): cell culture | mammalian: suitable. impurities: HIV, hepatitis B and hepatitis C, none detected. solubility: 0.5 M acetic acid: 1 . mg/mL. suitability: suitable for substrate for collagenase. web metadata keyword.default: cell attachment substrate, epithelial cell attachment factor, endothelial cells attachment factor, myocyte attachment factor, neural cell attachment factor. UniProt accession no.: P08572, P29400, P53420, Q01955, Q14031. application(s): diagnostic assay manufacturing. storage temp.: −. 20°C. Gene Information: human ... COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288). Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).