Well-Coated Neutravidin, 96 well plate, Black, 5 Plates

Well-Coated TM NeutravidinTM plates are designed to specifically bind biotinylated molecules, including biotin tagged antibodies, with minimal non-specific binding. This is particular advantageous for antibodies known to denature upon direct binding to polystyrene plates. Biotin exhibits an extraordinary binding affinity for avidin (Ka=10 15 M -1 ) and NeutravidinTM (Ka=10 15 M -1 ). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by autoclaving. NeutravidinTM is in many respects is similar to avidin except that it has no carbohydrate side chains to eliminate lectin binding; is of near neutral pI (6.3) to reduce non-specific adsorption; lacks the RYD sequence eliminating interaction with RGD domain of adhesion receptors. The binding of NeutravidinTM is similar to that of avidin and streptavidin with less non-specific binding. Well-Coated TM NeutravidinTM plates are suitable for direct, indirect, competitive and sand wich assays. The wells are coated to a 100μl depth and are supplied pre-blocked in our proprietary SuperiorTM Blocking Buffer . The clear, white and black plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively. Features High binding capacity for biotin Low non-specific binding Ideal for peptides, antibodies and small hydrophilic molecules Reduced non-specific binding as plates are pre-blocked with SuperiorTM Blocking Buffer