CHOLESTEROL-WATER SOLUBLE BIOREAGENT SUITABLE FOR CELL CULTURE POWDER

Application

Cholesterol-Water Soluble has been used:

• in free cholesterol (FC) overloading of rat smooth muscle cells (SMCs)
• in cytotoxicity assay
• for the incubation of CD4⁺ T cells with Helicobacter pylori
• to study the membrane cholesterol level in SH-SY5Y human neuroblastoma cells
• to study the effects of cholesterol on cell viability and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction in primary cultured rat hippocampal neurons
• as a supplement for growth of bacterial culture
• to examine the interaction of S protein with angiotensin-converting enzyme 2 (ACE2)
• to investigate the effects of cholesterol on potassium currents in the inner hair cells of guinea pigs
• for reversible inhibition of proteinase K

Packaging

Package size based on cholesterol

Other Notes

Contains approx. 40 mg of cholesterol per gram; balance methyl-β-cyclodextrin.

Preparation Note

Preparea solution at 300mg/mL in water. Let thesolution sit undisturbed for one hour after diluting to obtain completesolubilization of product.

biological source: ovine wool. Quality Level: 200. product line: BioReagent. form: powder. technique(s): cell culture | mammalian: suitable. solubility: H₂O: 300 . mg/mL. web metadata keyword.default: Sigma-Aldrich C4951, Cholesterol-Water Soluble powder, BioReagent, suitable for cell culture, Cholesterol−. methyl-β. -cyclodextrin, cholesterol cyclodextrin complex. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).