CHOLERA TOXIN B SUBUNIT RECOMBINANT EXPRESSED IN HEK 293 CELLS

General description

The advantage of this product over the native CTXB is that it has no traces of Vibrio cholera, and no contamination of the highly toxic Cholera toxin A subunit. This product is expressed in human HEK 293 cells as a C-terminally his-tagged protein with a calculated molecular mass of 13 kDa (amino acids Thr22-Asn124).

Application

Cholera Toxin B subunit has been used:

• for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
• in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Reconstitution

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 1X PBS

Analysis Note

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with ≤1 μg of Cholera toxin B subunit per mL.