CHLORODICYCLOHEXYLPHOSPHINE 97%

General description

A cell-permeable diamino compound that selectively blocks nuclear translocation of NF-κB p65 and its transcription activity (IC₅₀ = 7.1 µM in a NF-κB reporter assay using RAW 264.7) without affecting IκB degradation. Shown to suppress DNA-binding of NF-κB and downregulate LPS-induced gene expression and apoptotic chromatin condensation.

A cell-permeable, selective blocker of the nuclear translocation of NF-κB p65 that does not affect IκB degradation (IC₅₀ = 7.1 µM in LPS-stimulated macrophages RAW 264.7 stably transfected with pNF-κB-SEAP-NPT). Shown to suppress DNA-binding of NF-κB and downregulate LPS-inducible expression of TNF-α, IL-1β, IL-6, iNOS, and COX-2. Also prevents LPS-induced apoptotic chromatin condensation.

Biochem/physiol Actions

Primary Target
Blocks nuclear translocation of NF-κB p65 and its transcription activity

Target IC₅₀: 7.1 µM in a NF-κB reporter assay using RAW 264.7

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Shin, H.M., et al. 2004. FEBS Lett.571, 50.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany