CHAPS SOLUTION

General description

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.

Application

CHAPS 100 mM solution has been used as a component of lysis buffer for the extraction of proteins from goat endometrial epithelial cells (EECs) and liver tissue.

Biochem/physiol Actions

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes). CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.

Molecular Weight: 614.88. Empirical Formula: C₃₂H₅₈N₂O₇S. description: zwitterionic. Quality Level: 100. form: liquid. mol wt: 614.88 . g/mol. concentration: 100 . mM in H₂O. technique(s): protein quantification: suitable. CMC: 8 . mM. transition temp: cloud point >. 100 . °C. density: 1.01 . g/mL . at 20 . °C. storage temp.: 2-8°C. SMILES string: C[C@H](CCC(: O)NCCC[N+](C)(C)CCCS([O-])(: O): O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C. InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1. InChI key: UMCMPZBLKLEWAF-RFCNGIAKSA-N. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves.