CELL GROWTH DETERMINATION KIT MTT BASED

General description

Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.

Application

Cell Growth Determination Kit, MTT based has been used:

• to measure the cell viability of preosteoblastic cells
• in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
• to determine the cell viability of primary cervical epithelial cells

Packaging

Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.

packaging: pkg of 1 . kit. Quality Level: 200. technique(s): UV/Vis spectroscopy: suitable, protein quantification: suitable. A_max: 570 nm. application(s): cell analysis
detection. detection method: colorimetric. shipped in: dry ice. storage temp.: −. 20°C. Pictograms: GHS02,GHS07. Signal Word: Danger. Hazard Statements: H225 - H315 - H319 - H335 - H336. Precautionary Statements: P210 - P233 - P240 - P241 - P303 + P361 + P353 - P305 + P351 + P338. Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2. Storage Class Code: 3 - Flammable liquids. WGK: WGK 3. Flash Point(F): 53.6 °F - closed cup. Flash Point(C): 12 °C - closed cup.