CDP-STAR CHEMILUMINESCENT LIQUID SUBSTRATE

Application

CDP-Star^® is a sensitive, chemiluminescent substrate for alkaline phosphatase that allows for the rapid, reproducible detection of alkaline phosphatase-labeled molecules in Northern, Southern, and Western blotting applications. Detection of alkaline phosphatase-labeled molecules with CDP-Star is extremely sensitive as a result of low background luminescence coupled with high intensity and prolonged light output from the enzyme catalysis. Following exposure to alkaline phosphatase-labeled molecules, maximum light emission occurs at approximately 60 minutes and continues for up to 24 hours, allowing for multiple film exposures and/or the sensitive detection of targets present in small amounts. For convenience, CDP-Star is supplied as a 0.25 mM, ready-to-use aqueous solution (i.e. no dilution is necessary). CDP-Star functions on both neutral and positively-charged nylon, giving the reagent added application versatility.

Features and Benefits

CDP-Star^® is supplied as a 0.25 mM ready-to-use aqueous solution for use in a variety of membrane-based applications. CDP-Star functions on both neutrally-charged and positively-charged nylon, giving the reagent added application versatility.

Caution

Store at 2-8 Â°C protected from light.

Legal Information

CDP-Star^® is a trademark of Tropix, Inc. Bedford, MA, USA and covered under U.S. Patent 5,326,882.

CDP-Star is a registered trademark of Tropix, Inc.

Molecular Weight: 495.20. Empirical Formula: C₁₈H₁₉Cl₂O₇Na₂P. Assay: ≥. 75% (HPLC). Quality Level: 200. form: liquid. usage: sufficient for 20 . blots detection of alkaline phosphatase-labeled molecules (10 × 10 cm). concentration: 0.25 . mM in H₂O. storage temp.: 2-8°C. SMILES string: Cl[C@]1(C2)C[C@H]3C4(OOC4(C5: CC(OP([O-])([O-]): O): C(Cl)C: C5)OC)[C@@H]2C[C@@H](C1)C3. InChI: 1S/C18H21Cl2O7P.2Na/c1-24-18(14-6-13(2-3-15(14)19)25-28(21,22)23)17(26-27-18)11-4-10-5-12(17)9-16(20,7-10)8-11. . /h2-3,6,10-12H,4-5,7-9H2,1H3,(H2,21,22,23). . /q. 2*+1/p-2/t10-,11-,12+,16-,17?,18?. . . InChI key: UKWLRLAKGMZXJC-VCCHNUGISA-L. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.