CATHEPSIN K SUBSTRATE II FLUOROGENIC - CALBIOCHEM

General description

An internally quenched, highly cathepsin K-selective, fluorogenic peptide substrate that is efficiently cleaved by CatK (k_cat/K_m = 0.426 µM^-1 S^-1), but not by Cathepsins B/L/S/F/H/V. Studies using WT and CatK^-/- mice reveal that the substrate is not cleaved by other proteolytic enzymes present in fibroblast lysates.

An internally quenched, highly cathepsin K-selective, fluorogenic peptide substrate that is efficiently cleaved by CatK (k_cat/K_m = 0.426 µM^-1 S^-1), but not by Cathepsins B/L/S/F/H/V. Studies using WT and CatK^-/- mice reveal that the substrate is not cleaved by other proteolytic enzymes present in fibroblast lysates. Emission Max: 420 NM, Excitation Max: 320 nM.

An internally quenched, highly cathepsin K-selective, fluorogenic peptide substrate that is efficiently cleaved by CatK (k_cat/Km = 0.426 µM⁻¹ S⁻¹), but not by Cathepsins B/L/S/F/H/V.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Abz-His-Pro-Gly~Gly-Pro-Gln-EDDnp

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Lecaille, F., et al. 2003. Biochem. J.375, 307.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 918.91. Empirical Formula: C₄₀H₅₀N₁₄O₁₂. Quality Level: 100. Assay: ≥. 95% (HPLC). form: solid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, desiccated, protect from light. color: bright yellow. solubility: DMSO: 5 . mg/mL. fluorescence: λ. _ex . 320 . nm. λ. _em . 420 . nm. shipped in: wet ice. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.