CATHEPSIN B FROM BOVINE SPLEEN (C005B-089312)

General description

Cathepsin B is a lysosomal enzyme that comprises a light chain (Lys1–Arg49) and a heavy chain (Val50–Thr253). It belongs to cysteine family C1.

Application

Cathepsin B from bovine spleen has been used in cleavage assay before high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis. It has also been used in the enzyme inhibition assays with protease inhibitors RfIP1 and ruthenium metalloarenes.

Biochem/physiol Actions

Cathepsin B displays an endopeptidase and peptidyl dipeptidase activities. It may be associated with the pathophysiology of tumors. Cathepsin B is also regarded as a prominent protease in Leishmaniasis. It is overactivated in muscular dystrophy, pulmonary emphysema, and bone resorption.

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Unit Definition

One unit will hydrolyze 1 μmole of Z-lysine p-nitrophenyl ester per min at pH 5.0 at 25 Â°C.

Physical form

Lyophilized powder containing sodium phosphate, sodium chloride and ~6% EDTA as stabilizer.

biological source: bovine spleen. Quality Level: 200. form: lyophilized powder. specific activity: ≥. 10 . units/mg protein. composition: Protein, ≥. 30% biuret . UniProt accession no.: P07688. storage temp.: −. 20°C. Gene Information: cow ... CTSB(281105). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).