CASPASE 8 HUMAN RECOMBINANT C-TERMINAL HISTIDINE-TAGGED EXPRESSED IN E. COLI

General description

Human recombinant C−terminal histidine tagged caspase 8 is expressed as the truncated pro-caspase 8 (amino acids 213−496). It is a fully active protein consisting of 18 kDa and 12.4 kDa subunits; the 12.4 kDa subunit contains the histidine tag.

Biochem/physiol Actions

Caspase 8 is at the top of the hierarchy of the caspase cascade and is considered an initiator caspase. It exists in the cell as an inactive 55 kDa proenzyme. It is converted to the active form, consisting of 18 kDa and 11 kDa subunits, upon its recruitment to the cytoplasmic domain of activated death receptors such as CD95 or TNFR via the adapter protein FADD. The activation of the proenzyme is triggered by the protein′s aggregation, which leads to auto- or transprocessing. Caspase 8 activates downstream caspases (caspases 3, 6, and 7) that cleave key cellular substrates and lead to apoptotic death of the cells.

Unit Definition

One unit will cleave 1.0 nmol of Ac-Ile-Glu-Thr-Asp-pNA per min at pH 7.4 at 25 °C.

Physical form

Solution in 10% sucrose containing 20 mM Tris-HCl, 150 mM imidazole-HCl, pH 8.0, 20 mM 2-mercaptoethanol, 500 mM NaCl, 2.5 mM EDTA, and 0.1% CHAPS.

recombinant: expressed in E. coli (C-terminal histidine tagged). Quality Level: 200. Assay: ≥. 90% (SDS-PAGE). form: buffered aqueous solution. specific activity: ≥. 1,000 . units/mg protein. mol wt: ~30 . kDa. UniProt accession no.: Q14790. shipped in: dry ice. storage temp.: −. 70°C. Gene Information: human ... CASP8(841). Pictograms: GHS07,GHS08. Signal Word: Danger. Hazard Statements: H315 - H317 - H319 - H360D. Precautionary Statements: P202 - P261 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313. Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - Skin Sens. 1. Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.