CASPASE-3 ASSAY KIT COLORIMETRIC

General description

Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.

The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (ε_mM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).

Application

The assay can be performed (a) in 1 mL volume and measured using a spectrophotometer, or (b) in 100 µL volume in a 96-well plate using an ELISA reader.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.

Other Notes

Use 17 megohm water only

usage: sufficient for 1,000 . multiwell tests (in 96-well multiwell plates), sufficient for 100 . standard tests (1 ml). Quality Level: 300. shipped in: dry ice. storage temp.: −. 20°C. Gene Information: human ... CASP3(836). Pictograms: GHS06,GHS08. Signal Word: Danger. Hazard Statements: H301 + H311 + H331 - H373 - H412. Precautionary Statements: P273 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P311 - P314. Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2. Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects. WGK: WGK 3. Flash Point(F): 415.4 °F - closed cup. Flash Point(C): 213 °C - closed cup.