General description
Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.
Application
Casein Blocking Buffer 10x has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemistry
Features and Benefits
- Better blocking performance than dried milk or BSA
- Easy-to-use concentrate
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent and colorimetric detection assays
Preparation Note
Dilute 10x Blocking Buffer with molecular biology grade water before use.
grade: for molecular biology. Quality Level: 200. form: solution. technique(s): Southern blotting: suitable. storage temp.: room temp. Pictograms: GHS07,GHS09. Signal Word: Warning. Hazard Statements: H315 - H317 - H319 - H411. Precautionary Statements: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338. Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51142508
- Condition:
- New
- HazmatClass:
- No
- MPN:
- B6429-500ML
akash.verma@cenmed.com
(732) 447-1115





