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carboxy-terminal flag-bap(tm) fusion protein

PROTEIN CONTROL C O TERMINAL FLAG O BAP

Catalog No.
C005B-438428
Manufacturer No.
P7457-.1MG
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
MG

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General description Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).

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General description

Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).

Application

Carboxy-terminal FLAG-BAP™ Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).

Biochem/physiol Actions

The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.

Other Notes

Control protein

Physical form

Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2

Preparation Note

Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml

Legal Information

FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

UPC:
41106502
Condition:
New
HazmatClass:
No
MPN:
P7457-.1MG
Temperature Control Device:
Yes

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