CALPAIN INHIBITOR I (C005B-007654)

Application

Calpain Inhibitor I have been used:

• as a component of protease inhibitor cocktail for gel shift analysis
• for treating HeLa cell cultures
• for inhibition of 26 S proteasome in 26 S proteasome inhibition assays

Biochem/physiol Actions

Calpain Inhibitor I inhibits tumor necrosis factor-induced (TNF-induced) cleavage of Poly (ADP-ribose) polymerase (PARP) and calpastatin in apoptosis. It also inhibits oxygen/glucose deprivation (OGD) induced cleavage of caspase-12 and Bcl-xL..

Can initiate apoptosis in HL-60 cells but blocks dexamethasone-induced apoptosis in thymocytes and cycloheximide-induced apoptosis in metamyelocytes. Inhibits cyclin B degradation and arrests the cell cycle at G₁/S and at meta-/anaphase. Blocks induction of nitric oxide synthase by LPS in macrophages.

Molecular Weight: 383.53. Empirical Formula: C₂₀H₃₇N₃O₄. biological source: synthetic (organic). Quality Level: 200. Assay: ≥. 97% (TLC). form: powder. color: white. solubility: DMSO: soluble, ethanol: soluble. web metadata keyword.default: Ac-LLnL-CHO. ALLN. MG-101. N-Acetyl-Leu-Leu-Norleu-al. N-Acetyl-L-leucyl-L-leucyl-L-norleucinal. Ac-LL-Norleucinal, calpain inhibitor I. calpain. cathepsin inhibitor. cathepsin B. cathepsin L. Ub-proteosome. ubiquinin. cysteine. nitric oxide production. storage temp.: −. 20°C. SMILES string: [H]C(: O)[C@H](CCCC)NC(: O)[C@H](CC(C)C)NC(: O)[C@H](CC(C)C)NC(C): O. InChI: 1S/C20H37N3O4/c1-7-8-9-16(12-24)22-19(26)18(11-14(4)5)23-20(27)17(10-13(2)3)21-15(6)25/h12-14,16-18H,7-11H2,1-6H3,(H,21,25)(H,22,26)(H,23,27)/t16-,17-,18-/m0/s1. InChI key: FMYKJLXRRQTBOR-BZSNNMDCSA-N. Gene Information: human ... CAPN1(823), CTSB(1508), PSMA1(5682). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).