CALCEIN AM 4MM IN ANHYDROUS DMSO

Application

In cells, calcein AM solution has been used in calcein-AM dye transfer assay. It has been used for the staining of cells to study quantification of cell-migration. It has been used to study cytotoxic effects of drugs in cells.

Biochem/physiol Actions

Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.

Fluorescent cell permeable derivative of calcein.

Physical form

Solution in 0.4% Calcein AM and 99.6% DMSO.

Molecular Weight: 994.86. Empirical Formula: C₄₆H₄₆N₂O₂₃. Quality Level: 100. Assay: ≥. 90% (HPLC). form: solution. concentration: 4 . mM in DMSO. λ. _max: 491-497 nm. application(s): diagnostic assay manufacturing
hematology
histology. storage temp.: −. 20°C. SMILES string: CC(: O)OCOC(: O)CN(CC(: O)OCOC(C): O)Cc1c(OC(C): O)ccc2c1Oc3c(CN(CC(: O)OCOC(C): O)CC(: O)OCOC(C): O)c(OC(C): O)ccc3C24OC(: O)c5ccccc45. InChI: 1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3. InChI key: XKFSBWQWNMZWFA-UHFFFAOYSA-N. Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): 185.0 °F - closed cup. Flash Point(C): 85 °C - closed cup. Personal Protective Equipment: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US).