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cad

CAD

Catalog No.
C005B-244990
Manufacturer No.
08100805-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VL

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Cell Line Origin Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumour Cell Line Description CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse

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Cell Line Origin

Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumour

Cell Line Description

CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse carrying wild-type SV40 T antigen under transcriptional control of rat tyrosine hydroxylase promoter. CAD has lost the immortalising oncogene and expresses neurone-specific proteins and synaptic vesicle proteins. CAD will differentiate to form neuronal processes in serum free conditions.

Application

Study of neuronal differentiation

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 8% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 X 104 cells/cm2 Cells should be dislodged by gentle pipetting; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

UPC:
42261808
Condition:
New
HazmatClass:
Yes
MPN:
08100805-1VL
Temperature Control Device:
Yes

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