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c3h/mca clone 16 mouse c3h embryo (c15-1311-867)

C3H/MCA CLONE 16 MOUSE C3H EMBRYO (C005B-235855)

Catalog No.
C005B-235855
Manufacturer No.
90110523-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI

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Cell Line Origin Mouse C3H embryo Cell Line Description Derived by exposing C3H/10T1/2 clone 8 cells with 3-methylcholanthrene for 24 hours. Subsequently they lost the property of contact inhibition.

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Cell Line Origin

Mouse C3H embryo

Cell Line Description

Derived by exposing C3H/10T1/2 clone 8 cells with 3-methylcholanthrene for 24 hours. Subsequently they lost the property of contact inhibition. Inoculation of 100,000 cells or more into syngenic mice has been reported to produce fibrosarcoma.

Application

Tumourigenicity studies.

Culture Medium

BME (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: mouse embryo. growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.
UPC:
41106101
Condition:
New
HazmatClass:
No
MPN:
90110523-1VL
Temperature Control Device:
Yes

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