BUFFERS IN A BOX PREMIX SSC BUFFER(20X)

General description

20x solution

Application

Buffers in a Box, Premixed SSC Buffer, 20x has been used:

• as a wash buffer in in situ hybridization to determine the expression of miRNAs in paraffin-embedded tissue specimens
• as a wash buffer in single-molecule fluorescent in situ hybridization (smFISH) protocol using U2-OS cells and primary neuronal cultures
• as a component of rolling-circle amplification (RCA) hybridization buffer for use in fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissue samples

Features and Benefits

• Stable 20x solution of saline sodium citrate (SSC) purified by filtration using a 0.2 μm pore size membrane
• resistant to enzymatic and nonenzymatic degradation
• free from nuclease or protease activity
• can store at room temperature

Quality

Absence of contaminants: Free of DNase and RNase according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

form: solution. packaging: pkg of 4 . L. manufacturer/tradename: Roche. pH: 7.0. shipped in: ambient. storage temp.: 20-25°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): does not flash. Flash Point(C): does not flash.