BRUCELLA AGAR FOR MICROBIOLOGY

Application

For the isolation and cultivation of Brucella species, in particular the pathogens B. melitensis, B. abortus and B. suis, from clinical material and comestibles of animal origin.

Preparation Note

Dissolve 41 g in 1 litre distilled water and autoclave at 121°C for 15 minutes. Let cool to 45-50°C and add the supplement according the data sheet.

Supplements :
Brucella differential agar:
1 ml/l 2% basic fuchsin solution (Cat. No. 03968). or 2% solution from thionine acetate (Cat. No. 88930)

Brucella selective agar:
500 mg/l Bacitracin (Cat. No. 11702)
1 mg/l Polymyxin B sulfate (Cat. No. P4932)
100 mg/l Cycloheximide (Cat. No. 01810)
(and if required) 1.25 mg/l ethyl violet (Cat. No. 228842)

For isolation of Campylobacter species:
50 ml/l heat inactivated horse serum (Cat. No. H1138)
250mg/l Sodium pyruvate (Cat. No. P2256)
250mg/l Sodium metabisulfite (Cat. No. 31448)
250mg/l Ferrous sulfate (Cat. No. 31236)
50-100 ml/l defibrinated sheep blood

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility: non-sterile. Quality Level: 200. form: powder. shelf life: limited shelf life, expiry date on the label. composition: agar, 13 . g/L , casein peptone, 10 . g/L , D(+)-glucose, 1 . g/L , meat peptone, 10 . g/L , sodium chloride, 5 . g/L , yeast extract, 2 . g/L . manufacturer/tradename: NutriSelect^®. Basic. technique(s): microbiological culture: suitable. final pH: 7.0±0.2 (25 . °C). application(s): cleaning products
clinical testing
cosmetics
environmental
food and beverages
personal care
veterinary, microbiology. suitability: Haemophilus spp., Pneumococcus spp., nonselective for Brucella spp., nonselective for Listeria spp., nonselective for Neisseria spp., nonselective for Streptococcus spp.. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H317. Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364. Hazard Classifications: Skin Sens. 1. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).