BROMPHENOL BLUE SODIUM MOLECULAR BIOLOGY REAGENT (C005B-034401)

General description

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Reconstitution

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Molecular Weight: 691.94. Empirical Formula: C₁₉H₉Br₄NaO₅S. form: powder. Quality Level: 200. technique(s): electrophoresis: suitable. solubility: water: soluble. suitability: suitable for nucleic acid electrophoresis. storage temp.: room temp. SMILES string: [Na+].Oc1c(Br)cc(cc1Br)C(: C2/C: C(Br)C(: O)C(Br): C2)\c3ccccc3S([O-])(: O): O. InChI: 1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28. /h1-8,24H,(H,26,27,28). /q. +1/p-1. InChI key: TWKDJMRHSLWRFI-UHFFFAOYSA-M. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).