BROMOBIMANE SUITABLE FOR FLUORESCENCE BIOREAGENT >= 95% (HPCE) (C005B-058527)

General description

Bromobimane, also called probe for thiols, is a fluorescent reagent activated upon a photolysis reaction. Bromobimane in solution reacts with small thiol groups (e.g., GSH) and with reactive protein thiol groups (e.g., hemoglobin). The reaction of Bromobimane with thiols is of second-order and dependent on pH. And, upon reacting with thiolate, it activates the water-soluble fluorescent product for detection.

Application

Bromobimane is used for the determination of thiols by the HPLC method. It is suitable as a pre-column derivatization agent for fluorometric determination of 2,3-dimercaptopropane-1-sulfonic acid and other dithiols. Bromobimane has been used as a fluorescent label in studying oligomycin-sensitive ATPase from beef heart mitochondria.

Features and Benefits

The benefits of using Bromobimane for the analysis of low molecular weight thiols are: Highly selective.

• Rapid reactivity.
• Ease of separation of derivatives by reverse HPLC.
• Ability to penetrate cells.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Fluorescent thiol-specific reagent

Molecular Weight: 271.11. Empirical Formula: C₁₀H₁₁BrN₂O₂. product line: BioReagent. Quality Level: 100. Assay: ≥. 95% (HPCE). form: solid. mp: 152-154 . °C (lit.). solubility: DMF: soluble, DMSO: soluble, acetonitrile: soluble, methanol: soluble. fluorescence: λ. _ex . 390 . nm. λ. _em . 478 . nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione), λ. _ex . 398 . nm. suitability: suitable for fluorescence. storage temp.: 2-8°C. SMILES string: CC1: C(C)C(: O)N2N1C(CBr): C(C)C2: O. InChI: 1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3. InChI key: AHEWZZJEDQVLOP-UHFFFAOYSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).