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brl-1 rat epithelial cells (c15-1314-278)

BRL-1 RAT EPITHELIAL CELLS (C005B-238162)

Catalog No.
C005B-238162
Manufacturer No.
94121649-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI

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Cell Line Origin Rat epithelial cells Cell Line Description Isolated by Dr Hayden Coon. Flat epithelial cell colonies that appear upon low density plating as suspensions of collagenase dissociated buffalo rat liver.

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Cell Line Origin

Rat epithelial cells

Cell Line Description

Isolated by Dr Hayden Coon. Flat epithelial cell colonies that appear upon low density plating as suspensions of collagenase dissociated buffalo rat liver. Cell origin not clearly defined.

Application

Feeder cells for co-culture with freshly isolated hepatocytes.

Culture Medium

Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Subculture Routine

Spit confluent cultures 1:100 i.e. seeding at 1x1,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: rat liver. growth mode: Adherent. karyotype: Diploid or pseudodiploid. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.
UPC:
12141915
Condition:
New
HazmatClass:
No
MPN:
94121649-1VL
Temperature Control Device:
Yes

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