COOMASSIE(R) BRILLIANT BLUE G 250 (C005B-052158)

General description

Brilliant Blue G (BBG) is a dye. It has been proposed as a substitute for internal limiting membrane (ILM) peeling.

Application

Brilliant Blue G was used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery.

Biochem/physiol Actions

P2X7 purinergic receptor antagonist.

Analysis Note

λ_max. ∼610 nm; E(1%,1 cm) ∼500-700 (H₂O)

Other Notes

Protein stain for gel electrophoresis. Does not penetrate the gel and is three times more sensitive than Naphthol Blue Black B; Simple and sensitive protein assay; Rapid, sensitive and versatile assay for proteins; free amino acids and several chemicals that interfere with the Lowry procedure cause no interference.

Legal Information

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Molecular Weight: 854.02. Empirical Formula: C₄₇H₄₈N₃NaO₇S₂. grade: for microscopy. Quality Level: 200. form: solid. SMILES string: [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(: O): O): C5\C: CC(\C: C5C): [N+](\CC)Cc6cccc(c6)S([O-])(: O): O)cc1. InChI: 1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56. /h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56). /q. +1/p-1. InChI key: RWVGQQGBQSJDQV-UHFFFAOYSA-M. Storage Class Code: 11. 13 - Combustible Solids. Non Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).