BRD0539 (C007B-068184)

InformationBRD0539 BRD0539 is a potent inhibitor of Streptococcus pyogenes Cas9 (SpCas9) with IC50 of 22 μM in an in vitro DNA cleavage assay.TargetsSpCas9 (Cell-free assay) 22 μMIn vitroBRD0539 is stable in human plasma and reversibly inhibits SpCas9. BRD0539 does not interfere with the SpCas9:gRNA interaction. BRD0539 dose-dependently blocks the formation of the DNA-bound state in a dose-dependent fashion. BRD0539 impairs the perturbation induced by the 4PAM DNA. BRD0539 is able to inhibit SpCas9 in the eGFP-disruption assay.Cell Research(from reference)Cell lines：U2OS.eGFP.PEST cells Concentrations：15 μM Incubation Time：2–24 h.

Specifications and Purity: ≥98%

Molecular Formula: C25H25FN2O3S

Molecular Weight: 452.54

PubChem CID: 54666326

Isomeric SMILES: CC1=CC=C(C=C1)S(=O)(=O)N2CC[C@H]3[C@@H]2C4=C(C=CC(=C4)C5=CC=CC=C5F)N[C@H]3CO