BONE MORPHOGENETIC PROTEIN 4 HUMAN (C005B-306351)

General description

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β (transforming growth factor β) superfamily of structurally related signaling proteins. Escherichia coli derived BMP-4 is a fully active homodimeric protein consisting of two 106 amino acid subunits which correspond to amino acids 303-408 of the full length BMP-4 precursor.

Application

BMP-4 human has been used for in vitro chondrogenesis in goat adipose tissue-derived mesenchymal stem cells.

Biochem/physiol Actions

Members of TGF-β (transforming growth factor β) superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life. Like BMP-7, BMP-4 (bone morphogenetic protein 4) is involved in the development and maintenance of bone and cartilage. Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. BMP-4 controls the expression of miRNAs in breast cancer cell lines, thereby regulating the proliferation and migration phenotypes. It is involved in carcinogenesis and exhibits its effect through the SMAD-dependent signaling. In mice, homozygous mutations in BMP4 cause early embryonic lethality.

Sequence

KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening.  Reconstitute in 5 mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/ml.  Do not vortex.  This solution can be stored at 2-8° C for up to 1 week.  For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20° C to -80° C.