BLEOMYCIN SULFATE FROM STREPTOMYCES VERTICILLUS FOR FLUORESCENCE MIXTURE OF BLEOMYCIN SULFATE SALTS LYOPHILIZED POWDER WHITE TO (C005B-089262)

General description

Chemical structure: glycopeptide

Application

Bleomycin sulfate is used to study tumor-specific cytotoxic activity in certain cell lines such as human normal oral cells(gingival fibrobast HGF, pulp cell HPC and periodontal ligament fibroblast HPLF), human oral squamous cell carcinoma (HSC-2, HSC-3, HSC-4, Ca9-22 and NA) and human promyelocytic leukemia HL-60 cells. It is used to study free radical-mediated mechanisms of DNA strand scission/breaking and as a transformed cell selection agent, especially for plant transformants. Recommended for use as a selection agent at 10-100 μg/ml.

Biochem/physiol Actions

Bleomycin sulfate binds to DNA, causes ssDNA scission at specific base sequences and inhibits DNA synthesis. This inhibitory action requires bleomycin to bind oxygen and a metal ion. It can also cleave RNA, to a lesser degree but more selectively. It acts as an inducer and regulator of apoptosis and inhibits tumor angiogenesis.

Packaging

Packaged in a bottomless glass bottle with its contents inside an inserted fused cone.

Components

This product is a mixture of glycopeptides antibiotics containing Bleomycin A₂ (70%) and B₂(30%). Bleomycins differ from each other in the terminal amine and show varying biological activity.

Caution

This product should be stored at 2-8°C.

Preparation Note

The product is soluble in water in water at 20 mg/mL and is active for several days. A solution of 2 units/mL in 0.1 M neutral potassium phosphate should be used within 14 days. A solution is active for three months at 3 units/mL in normal saline.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

biological source: Streptomyces verticillus. Quality Level: 200. grade: for fluorescence. form: powder or crystals. quality: lyophilized. color: white to off-white. solubility: H₂O: 20 . mg/mL, clear, colorless. cation traces: Cu: ≤. 0.1%. antibiotic activity spectrum: Gram-negative bacteria, Gram-positive bacteria, neoplastics. Mode of action: DNA synthesis | interferes. storage temp.: 2-8°C. SMILES string: [O-]S([O-])(: O): O.C[C@@H](O)[C@@H](NC(: O)[C@@H](C)[C@H](O)[C@@H](C)NC(: O)[C@H](NC(: O)c1nc(nc(N)c1C)[C@H](CC(N): O)NC[C@H](N)C(N): O)[C@@H](O[C@@H]2O[C@@H](CO)[C@@H](O)[C@H](O)[C@@H]2O[C@@H]3O[C@H](CO)[C@@H](O)[C@@H](OC(N): O)[C@@H]3O)c4c[nH]cn4)C(: O)NCCc5nc(cs5)-c6ncc(s6)C(: O)NCCC[S+](C)C.C[C@@H](O)[C@@H](NC(: O)[C@@H](C)[C@H](O)[C@@H](C)NC(: O)[C@H](NC(: O)c7nc(nc(N)c7C)[C@H](CC(N): O)NC[C@H](N)C(N): O)[C@@H](O[C@@H]8O[C@@H](CO)[C@@H](O)[C@H](O)[C@@H]8O[C@@H]9O[C@H](CO)[C@@H](O)[C@@H](OC(N): O)[C@@H]9O)c%10c[nH]cn%10)C(: O)NCCc%11nc(cs%11)-c%12ncc(s%12)C(: O)NCCC[S+](C)C. InChI: 1S/2C55H83N17O21S3.H2O4S/c2*1-20-33(69-46(72-44(20)58)25(12-31(57)76)64-13-24(56)45(59)82)50(86)71-35(41(26-14-61-19-66-26)91-54-43(39(80)37(78)28(16-73)90-54)92-53-40(81)42(93-55(60)88)38(79)29(17-74)89-53)51(87)67-22(3)36(77)21(2)47(83)70-34(23(4)75)49(85)63-10-8-32-68-27(18-94-32)52-65-15-30(95-52)48(84)62-9-7-11-96(5)6. 1-5(2,3)4/h2*14-15,18-19,21-25,28-29,34-43,53-54,64,73-75,77-81H,7-13,16-17,56H2,1-6H3,(H13-,57,58,59,60,61,62,63,66,67,69,70,71,72,76,82,83,84,85,86,87,88). (H2,1,2,3,4)/t2*21-,22+,23+,24-,25-,28-,29+,34+,35+,36-,37+,38+,39-,40-,41-,42+,43-,53-,54-. /m00./s1. InChI key: OOXTWFJZZAJGKA-CNLAFNBISA-N. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H340 - H351 - H361fd. Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501. Hazard Classifications: Carc. 2 - Muta. 1B - Repr. 2. Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges.