BISBENZIMIDE H 33342 FOR FLUORESCENCE (C005B-083506)

Application

bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP.

Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

Biochem/physiol Actions

Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Suitable for vital DNA staining of a variety of cell types; As a probe of membrane permeability in mammalian cells

Molecular Weight: 561.93 (anhydrous basis). Empirical Formula: C₂₇H₂₈N₆O · 3HCl · xH₂O. grade: for fluorescence. Quality Level: 200. Assay: ≥. 97.0% (HPLC). fluorescence: λ. _ex . 340 . nm. λ. _em . 510 . nm, λ. _ex . 355 . nm. λ. _em . 465 . nm in TE buffer. DNA. storage temp.: 2-8°C. SMILES string: Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2: NCc3cc(ccc3N2)C4: NCc5cc(ccc5N4)N6CCN(C)CC6. InChI: 1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35. . . /h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33). 3*1H. InChI key: FYEVKHPLBHLWHK-UHFFFAOYSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves.