BILIVERDIN REDUCTASE A HUMAN RECOMBINANT EXPRESSED IN ESCHERICIA COLI

Biochem/physiol Actions

Biliverdin reductase catalyzes the transformation of the blue-green pigment biliverdin IX to the yellow-orange bile pigment bilirubin IX by converting a double-bond between the second and third pyrrole ring into a single-bond. This enzyme has two distinct cofactor-dependent pH optima. In the acidic range of pH 6.0-6.7, NADH is utilized, whereas in the alkaline range of pH 8.5-8.7, NADPH is utilized. Biliverdin reductase is considered a major physiologic cytoprotectant. Biliverdin reductase suppresses experimental autoimmune encephalomyelitis in rats. Depletion of biliverdin reductase leads to accumulation of cellular oxidants and augmented cell death.

Unit Definition

1 unit of biliverdin reductase will trans­form 1 nanomole of biliverdin to bilirubin per minute in an NADPH dependent reaction at pH 8.5 using 100 mM K-phosphate buffer at 37 °C.

Physical form

Solution containing 20 mM potassium phosphate buffer, 10% glycerol, 0.2% Igepal CA630, 1 mM EDTA, and 0.1 mM DTT.

recombinant: expressed in E. coli. Quality Level: 200. Assay: ≥. 90% (SDS-PAGE). form: buffered aqueous solution. specific activity: ≥. 700 . units/mg protein. shipped in: dry ice. storage temp.: −. 20°C. Gene Information: human ... BLVRA(644). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.