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bicinchoninic acid kit for protein determination

BICINCHONINIC ACID PROTEIN DETERMINATION KIT

Catalog No.
C005B-101299
Manufacturer No.
BCA1-1KT
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
KT

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Application Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells. Features and Benefits Simple, sensitive colorimetric assay for proteins Two stable reagents

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Application

Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells.

Features and Benefits

  • Simple, sensitive colorimetric assay for proteins
  • Two stable reagents are used for the working solution
  • Faster and easier than the Lowry protein assay
  • Compatible with many detergents both ionic and nonionic
  • Less variation between different proteins than the Bradford dye-binding assay
  • Adaptable for use in microwell plates

Principle

Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.

Quality Level: 200. usage: sufficient for ~500 . assays. storage temp.: 2-8°C. Pictograms: GHS05,GHS09. Signal Word: Danger. Hazard Statements: H290 - H318 - H410. Precautionary Statements: P234 - P273 - P280 - P305 + P351 + P338 - P390 - P391. Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1. Storage Class Code: 8A - Combustible, corrosive hazardous materials. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type ABEK (EN14387) respirator filter.
UPC:
41106511
Condition:
New
HazmatClass:
No
MPN:
BCA1-1KT

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