BETACELLULIN HUMAN RECOMBINANT

Application

This product has been used to study differentiation of pancreatic acinar cell line (AR42J) into insulin-secreting cells and has been linked to a role in the growth and differentiation of islet cells in human pancreas.

Biochem/physiol Actions

Betacellulin, and the other members of the EGF family of growth factors, binds with high affinity to both EGFR and to erbB-4. Overexpression of Betacellulin with erbB-3 and erbB-4 has been linked to human endometrial adenocarcinoma.

Caution

This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.

Preparation Note

This product was lyophilized from a 0.2 μm filtered solution of PBS containing 5 mg of bovine serum albumin. It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 1 μg/mL.

Analysis Note

The biological activity of this product is measured by an ability to stimulate H-thmidine incorporation in EGF-responsive mouse BALB/c 3T3 fibroblasts. The EC₅₀ is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

biological source: human. Quality Level: 100. recombinant: expressed in E. coli. Assay: ≥. 97% (SDS-PAGE). form: lyophilized powder. potency: 0.1-0.6 . ng/mL ED₅₀. mol wt: protein 9.5 . kDa. packaging: pkg of 10 . μ. g. storage condition: avoid repeated freeze/thaw cycles. technique(s): cell culture | mammalian: suitable. impurities: endotoxin, tested. web metadata keyword.default: Differentiation factor, AR42J, insulin-secreting cell inducer, insulin cell differentiation factor. UniProt accession no.: P35070. storage temp.: −. 20°C. Gene Information: human ... BTC(685). Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).