BETA-ENDOTHELIAL CELL GROWTH FACTOR HUMAN ?ETA-ECGF RECOMBINANT EXPRESSED IN E. COLI SUITABLE FOR CELL CULTURE

General description

The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor, and is mapped to human chromosome 11q13.1. Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa.

Application

β-Endothelial Cell Growth Factor human has been used:

• in cell death assay
• in endothelial cell culture
• in human umbilical vascular endothelial cell (HUVEC) culture

Biochem/physiol Actions

β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism. This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy, and polycystic ovary syndrome. It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor.

Other Notes

14 amino acid, N-terminal extended form of human FGF-acidic.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Analysis Note

The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.

biological source: human. Quality Level: 200. recombinant: expressed in E. coli. Assay: ≥. 97% (SDS-PAGE). form: lyophilized powder. potency: 0.03-0.3 . ng/mL ED₅₀/EC₅₀. quality: endotoxin tested. mol wt: predicted mol wt ~17 . kDa. packaging: pkg of 25 . μ. g. storage condition: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.). technique(s): cell culture | mammalian: suitable. impurities: ≤. 1.0 . EU/μ. g protein Endotoxin level (LAL test). UniProt accession no.: P49765. storage temp.: −. 20°C. Gene Information: human ... VEGFB(7423). Storage Class Code: 11 - Combustible Solids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).